Webinar: Evaluating CRISPR/Cas9 Off-Target Edits
Identify CRISPR/Cas9 Gene Editing Events with Single-Cell Whole Genome Sequencing
Over 90% of CRISPR off-target effects detected using ResolveDNA® were missed by prediction methodology. This reveals a gap in the methods used to detect and characterize actual CRISPR/Cas9 activity, with potential implications for validation of gene therapy candidates. Regulatory agencies are taking notice and drafting guidelines to navigate the development of cell and gene therapy products.
In this webinar, Dr. Victor Weigman, PhD, Sr. Director of Computational Biology at BioSkryb, discusses a comprehensive approach to characterizing gene editing events. He explores an innovative workflow that can better identify on- and off-target gene editing events in individual cells following CRISPR/Cas9 gene editing.
What you will learn:
- PTA chemistry, found in ResolveDNA, provides robust and precise whole genome sequencing data at the single-cell level.
- Off-target genetic changes following CRISPR/Cas9 gene editing are highly variable and extensive.
- Intercellular variability is common in on- and off-target editing events and should be considered when using bulk sequencing as a confirmation step.