ResolveDNA

dna-chain

ResolveDNA™ Whole Genome Amplification Kits overcome the inherent challenges of low coverage uniformity in single-cell genomics that lead to increased sequencing costs and complex data analysis.

The controlled reaction parameters employed in this PTA-based kit enables reproducible recovery of >95% of the genomes of single cells and limited DNA input samples with industry-leading uniformity and accuracy.

Key features and benefits of ResolveDNA Whole Genome Amplification Kits include:

  • Significantly lower allelic dropout and biases compared to existing WGA methods that yield low and variable coverage across the genome
  • Specific amplification of the primary template with >97% of reads mapping to the human genome and no detectable product in no template control reactions
  • Simple, user-friendly workflow that requires less than 45 mins of hands-on time
  • Consistent WGA fragment sizes and yields from picogram to nanogram inputs



sequencing-performance

ResolveDNA™ Whole Genome Amplification Kits offer superior coverage and uniformity in single-cell WGS.

Ten single cells were isolated from a human B-lymphocyte cell culture (CEPH1463/NA12878/ GM12878 human genome reference standard). WGA was performed on individual cells, using the ResolveDNA Whole Genome Amplification Kit. WGA products were converted to indexed libraries and subjected to high-coverage whole genome sequencing (WGS) on the Illumina® platform. For the other WGA methods, low-pass WGS data (generated from individual BJ1 fibroblasts), were obtained from a previously published study (Chen, C. et al. Science 2017; 356: 189).

To achieve a fair comparison of the various WGA methods, raw data for all samples were aligned and pre-processed for variant calling using the same pipeline. All metrics shown in the table were generated from randomly subsampled BAM files (300 million reads per cell). Note that the metrics for all the methods other than the ResolveDNA kit are overestimates, due to the way in which data analysis was performed in the original study.

Compatible with:

  • Single or multiple cells; ultra-low amounts of DNA (>4 pg—10 ng)
  • Any cell collection method, plate or tube format

Three easy steps: cell lysis, whole genome amplification,bead-based cleanup of amplified DNA

WGA products are converted to libraries for multiplexed Illumina® sequencing (no fragmentation required)

WGA-Workflow